Coding
HygR

Part:BBa_K2984070:Design

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-19)


Hygromycin B CDS; Selection for Transformation - Antibiotic resistance aphVII (hyg)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 779
    Illegal NgoMIV site found at 887
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was used to make our composite parts compatible for the iGEM registry. This part is not MoClo compatible because it does not have the MoClo overhangs!


Source

Berthold, et al. (2002)

References